Journal: Cell reports

Article Title: Blockade of MCU-Mediated Ca 2+ Uptake Perturbs Lipid Metabolism via PP4-Dependent AMPK Dephosphorylation

doi: 10.1016/j.celrep.2019.02.107

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti- PPX (C-6) , Santa Cruz Biotechnology Inc. , Cat# sc-374106.

Techniques: Diagnostic Assay, Recombinant, Electron Microscopy, Modification, Protease Inhibitor, Glo Assay, Activity Assay, Plasmid Preparation, Software

Journal: Cell reports

Article Title: Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression

doi: 10.1016/j.celrep.2024.113823

Figure Lengend Snippet: (A,B) Localization of Mira throughout the cell cycle in u6atacK01105 (u6atac−/−) mutant and U2A RNAi NBs. Inset, Mira localization at apical (yellow boxes) and basal (grey boxes) centrosomes; Scale bar, 5 μm; n ≥ 19 NBs. (C,D) Cortical localization of Mira in metaphase u6atac−/− NBs. Arrows, basal cortex. Scale bar, 5 μm; n ≥ 19 NBs. (E,F) Pros nuclear localization in u6atac−/−mutant NBs. Dashed circles outline NBs. Scale bar, 5 μm; n ≥ 6 brains. (G,H) Mitotic Index in u6atac−/− mutant brains. Yellow circles, mitotic NBs; Grey circles, non-mitotic NBs; Scale bar, 20 μm; n ≥ 7 brains. (I,J) Flfl staining in u6atac−/− mutant NBs. Dashed circles, NBs; Scale bar, 10 μm; n = 3 biological replicates. (K) PP4-19c staining in u6atac−/− mutant NBs. Dashed circles, NBs; Scale bar. (L) Localization of Kin17-HA in NBs during interphase and mitosis. Scale bar, 5 μm. (M) Quantitative RT-PCR results for Flfl transcript. Left panel, position of primers. n = 8 biological replicates (N) RNA immunoprecipitation of flfl mRNA by Kin17-HA in Drosophila embryos. Top panel, IP of Kin17-HA proteins; Bottom panels, flfl RT-PCR products obtained from cDNA libraries generated from RIP immunoprecipitates. Lysate and IP samples were run on the same blot but images were taken from different exposures. (O) aPKC and Pros RT-PCR results obtained from cDNA libraries generated from RIP immunoprecipitates. Data are presented as the mean ± 1 SD. n.s., not significant; *, p<0.05; **, p<0.01; ***, p<0.001, unpaired student’s t-test with Bonferroni correction (B). unpaired Student’s t-test for (F, H), and paired Student’s t-test for (J).

Article Snippet: Rabbit polyclonal PP4-19c (IP: 4 μg/mg; IF: 1:1000; IB: 1:1000) , Proteintech , #10262-1-AP.

Techniques: Mutagenesis, Staining, Quantitative RT-PCR, RNA Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction, Generated

Journal: Cell reports

Article Title: Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression

doi: 10.1016/j.celrep.2024.113823

Figure Lengend Snippet: (A,B) Relative Flfl levels in ctrl, Kin17 RNAi, and Kin17 rescue NBs. Dotted lines, NBs [identified by size and CD8-GFP expression (not shown)]; Scale bar, 5 μm; n = 3 biological replicates. (C,D) PP4-19c localization in Kin17 RNAi and flfln42 NBs. Dashed circles, NBs. Ase staining indicates nucleus. Scale bar, 5 μm; n ≥ 51 NBs. Data are presented as the mean ± 1 SD. *, p<0.05; ***, p<0.001, paired student’s t-test with the Bonferroni correction for (B) and unpaired student’s t-test with the Bonferroni correction for (D).

Article Snippet: Rabbit polyclonal PP4-19c (IP: 4 μg/mg; IF: 1:1000; IB: 1:1000) , Proteintech , #10262-1-AP.

Techniques: Expressing, Staining

Journal: Cell reports

Article Title: Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression

doi: 10.1016/j.celrep.2024.113823

Figure Lengend Snippet: (A,B) Localization of Mira in PP4-19c and Flfl RNAi NBs throughout the cell cycle. Inset, Mira localization at apical (yellow) and basal (grey) centrosomes; Scale bar, 5 μm; n ≥ 27 NBs. (C) IP of PP4-19c (left) and quantification of dephosphorylation of Mira phosho-Serine96 peptide by PP4 precipitates (right). n = 4 biological replicates. (D) Localization of RFP-Flfl in NBs. Arrowheads, mitotic spindle; Arrows, centrosome; Scale bar, 5 μm. (E,F) Localization of PP4-19c in ctrl and PP4-19c RNAi NBs. Inset, PP4-19c localization at apical (yellow) and basal (grey) centrosomes; Scale bar, 5 μm; n ≥ 61 NBs. (G,H) Localization of Mira in prophase NBs expressing V5-PACT or V5-Flfl-PACT. Inset, Mira localization at apical (yellow boxes) and basal (grey boxes) centrosomes; Scale bar, 5 μm; n ≥ 25 NBs. Data are presented as the mean ± 1 SD. n.s., not significant; *, p<0.05; **, p<0.01; ***, p<0.001, paired student’s t-test for (C), unpaired student’s t-test for (H), and unpaired Student’s t-test with the Bonferroni correction for (B, F). See also Figure S4.

Article Snippet: Rabbit polyclonal PP4-19c (IP: 4 μg/mg; IF: 1:1000; IB: 1:1000) , Proteintech , #10262-1-AP.

Techniques: De-Phosphorylation Assay, Expressing

Journal: Cell reports

Article Title: Kin17 regulates proper cortical localization of Miranda in Drosophila neuroblasts by regulating Flfl expression

doi: 10.1016/j.celrep.2024.113823

Figure Lengend Snippet: Key resources table

Article Snippet: Rabbit polyclonal PP4-19c (IP: 4 μg/mg; IF: 1:1000; IB: 1:1000) , Proteintech , #10262-1-AP.

Techniques: Binding Assay, Virus, Recombinant, Protease Inhibitor, Phosphatase Assay, SYBR Green Assay, One Step RT-PCR, Plasmid Preparation, Software, Microscopy, Membrane, Reverse Transcription, PCR Cloning